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This unit specifies the competencies required to apply entomological techniques. It involves determining classification of insects, demonstrating anatomy and physiology of insects, and determining insect ecology. It also involves carrying out rearing of insects, demonstrating pest control and management and determining control of arthropod vectors 

 

1️⃣ Introduction to Microbes

Microbiology is the study of microscopic living organisms called microbes. These include:

  • Bacteria – single-celled, prokaryotic organisms found everywhere

  • Viruses – non-living infectious particles that require a host cell to multiply

  • Fungi – yeasts and molds that can be unicellular or multicellular

  • Protozoa – single-celled eukaryotes found mostly in water

  • Algae – photosynthetic organisms found in aquatic environments

  • Helminths – parasitic worms, visible at adult stage but studied in microbiology due to their microscopic larvae

Microbes are important because they:

  • Support life (e.g., decomposition, nutrient recycling)

  • Help in food production (e.g., yoghurt, bread)

  • Are used in biotechnology and medicine

  • Can cause diseases (pathogens), affecting humans, animals, and plants


2️⃣ Laboratory Safety & Microscopy

Because microbes can be harmful, safety in the microbiology lab is crucial.

Lab Safety Rules

  • Wear personal protective equipment (coat, gloves, goggles)

  • Sterilize working area before and after experiments

  • Properly dispose of microbial waste (autoclaving)

  • Never eat, drink, or apply cosmetics in the lab

  • Handle cultures carefully to prevent contamination

Microscopy in Microbiology
The light microscope is most common for viewing bacteria, fungi, and protozoa. Key parts include:

  • Eyepiece & objective lenses – magnify the specimen

  • Stage – holds slide

  • Light source & condenser – illuminate the sample

  • Coarse and fine adjustment knobs – focus the image

Types of microscopy used:

  • Bright-field microscopy – basic staining and observation

  • Electron microscopy (TEM & SEM) – used for viruses and ultrastructure (very high magnification)

  • Fluorescence microscopy – uses fluorescent dyes to view specific structures


3️⃣ Microbial Growth

Microbial growth refers to an increase in number of cells, not cell size.

Requirements for Growth

  • Nutrients – carbon, nitrogen, minerals, etc.

  • Temperature – psychrophiles (cold), mesophiles (moderate), thermophiles (hot)

  • pH – acidophiles, neutrophiles, alkaliphiles

  • Oxygen – aerobes, anaerobes, facultative anaerobes

  • Moisture & osmotic balance

Binary Fission
Most bacteria multiply by binary fission, producing two identical cells.

Bacterial Growth Curve
In batch culture, bacteria pass through:

  1. Lag phase – adaptation, no rapid increase

  2. Log (exponential) phase – rapid cell division

  3. Stationary phase – nutrients limited, growth = death

  4. Death phase – cells die due to waste buildup & lack of nutrients

Culture Media

  • Liquid (broths) & solid (agar plates)

  • Can be selective, differential, or enriched depending on purpose